Optically active compounds with a chromophore close to a chiral center absorb differently left and right circularly polarized light, which can be detected with excellent sensitivity and selectivity. When chiral compounds are measured using a UV-Visible detector, the elution order of d- and l- enantiomers cannot be easily predicted even when separated by a chiral column.

The CD-4095 circular dichroism detector makes it possible to positively distinguish between the d- and l- enantiomers by measuring their positive and negative peaks. It is also possible to quantify the d- and l-forms even when they are not separated, a calibration curve based on the g-factor can be used to determine the enantiomeric ratio.

The ChromNAV CDS can be used to simultaneously measure the CD, g-factor data and UV-visible signal. The CD spectra of eluted compounds can be measured by triggering scanning manually, by timed program or automatically by threshold.

Specifications